Publications and Patents

In Revision/Preparation

Yaganoglu, S., Helassa, N., Gaub, B.M., Welling, M.A., Shi, J., Müller, D.J., Török, K., and Pantazis, P., GenEPi: Piezo1 based

fluorescent reporter for visualizing mechanical stimuli with high spatiotemporal resolution.

Invention filed: E.P. patent pending (2016, EP 2016/191538).

Sonay, A.Y., Yaganoglu, S., Konantz, M., Teulon, C., Jiang, S., Behzadi, S., Crespy, D., Landfester, K., Roke, S., Lengerke, C., and Pantazis, P., Biodegradable harmonophores for targeted high-resolution in vivo tumor imaging.

Invention filed: E.P. patent pending (2016, EP 2016/191538). Nominated for the SPARK Award 2017.

2020

Publications

Welling, M., Kalyviotis, K., Pantazis, P., Primed Track: reliable volumetric single-cell tracking and lineage tracing of living specimen with dual-labeling approaches.

Bio-protocol. (Accepted)

Kalyviotis, K., Qin, H., Pantazis, P., (2020) Chapter 19 - PhOTO zebrafish and primed conversion: advancing the mechanistic view of development and disease. In: Gerlai RT, editor. Behavioral and Neural Genetics of Zebrafish.

Academic Press. p. 309-22. doi:10.1016/B978-0-12-817528-6.00019-X

Malkinson, G., Mahou, P., Chaudan, É., Gacoin, T., Sonay, A. Y., Pantazis, P., Beaurepaire, E., Supatto, W., (2020) Fast In Vivo Imaging of SHG Nanoprobes with Multiphoton Light-Sheet Microscopy.

ACS Photonics 2020, 7 (4), 1036-1049.

2019

Publications

Welling, M.A., Mohr, M.A., Ponti, A., Sabater, L.R., Boni, A., Kitazawa, Y., Liberali, P., Peters, A., Pelczar, P., and Pantazis, P. (2019)

Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins.

eLife 8, doi:10.7554/eLife.44491

2018

Publications

Slenders, E., Bové, H., Urbain, M., Mugnier, Y., Sonay. A.Y., Pantazis, P., Bonacina, L., Vanden Berghe, P., vandeVen, M., and Ameloot, M., (2018) Image Correlation Spectroscopy with Second Harmonic Generating Nanoparticles in Suspension and in Cells.

J Phys Chem Lett. 20:6112-6118.

Sugiyama, N., Sonay, A.Y., Tussiwand, R., Cohen, B., and Pantazis, P., (2018) Effective Labeling of Primary Somatic Stem Cells with

BaTiO3 Nanocrystals for Second Harmonic Generation Imaging.

Small 8:1703386.

Mohr, M.A., and Pantazis, P., (2018) Primed conversion: the new kid on the block for photoconversion.

Chemistry 33:8268-8274.

Nienhaus, K., Mohr, M.A., Kobitski, A,Y., Sabater, L.R., Obara, C.J., Lippincott Schwartz, J., Nienhaus, G.U., and Pantazis, P. (2018)

Primed Green to Red Photoconversion of Fluorescent Proteins Occurs via a Triplet State.

Biophysical Journal 114:533a.

Patents

Pantazis, P., Dempsey, W.P., Truong, T.V., Fraser, S.E., and Georgieva, L. “Method And Device To Achieve Spatially Confined Photointeraction At The Focal Volume Of A Microscope” U.P. patent granted (15 May 2018, US 90,971,136).

Pantazis, P., Pu, Y., Hong, J., Psaltis, D., and Fraser, S.E. “Second Harmonic Imaging Nanoprobes And Techniques For Use Thereof” U.S. patent granted (20 Feb 2018, US 9,971,136).

2017

Publications

Nugraha, B., Mohr, M.A., Ponti, A., Emmert, M.Y., Weibel, F., Hoerstrup, S.P., Moll, S., Certa, U., Prunotto, M., and Pantazis, P., (2017)

Monitoring and manipulating cellular crosstalk during kidney fibrosis inside a 3D in vitro co-culture.

Scientific Reports 7:14490.

Mohr, M.A., Kobitski, A.Yu., Sabater, L.R. Nienhaus, K., Obara, C.J, Lippincott Schwartz, J., Nienhaus, G.U., and Pantazis, P., (2017)

Rational Engineering of Photoconvertible Fluorescent Proteins for Dual Color Fluorescence NanoscopyEnabled by a Triplet State Mechanism of Primed Conversion.

Angew. Chem. Int. Ed. 56:11628 11633.

Featured in: Bergman, F. (2017) Red fluorescence in two steps ETH Zurich News.

Invention filed: U.S. provisional patent submitted (2017, Application Serial No. 62/446,023).

Zhang,W., Lohman, A.W., Zhuravlova, Y., Lu, X., Wiens, M.D., Hoi, H., Yaganoglu, S.,Mohr, M.A., Kitova, E.N., Klassen, J.S., Pantazis, P., Thompson, J.R., and Campbell, R.E., (2017) Optogenetic control with a photocleavable protein.

Nature Methods 14:391–394.

Sonay, A.Y., and Pantazis, P., (2017) Bioinspired Second Harmonic Generation.

Proc. SPIE 10411, Clinical and Preclinical Optical Diagnostics,104110D.

Patents

Pantazis, P.and Yaganoglu, S., “Piezo1-based fluorescent reporter” E.P. patent pending (2017, EP 2017/17210479.6).

Mohr, M.A.,and Pantazis, P., “Engineered photoconvertible fluorescent proteins (pcFPs) for primed conversion” U.S. patent pending (2017, US 62/446,023).

2016

Publications

Mohr, M.A., Argast, P., and Pantazis, P., (2016) Labeling cellular structures in vivo using confined primed conversion of photoconvertible fluorescent proteins.

Nature Protocols 11:2419-2431.

Invention W.O. patent pending (2014, WO 2014/147211)

E.P. patent pending (2014, EP 2014/055669) licensed to: AHF Analysentechnik, Tübingen/Germany.

Welling, M., Ponti, A., and Pantazis, P., (2016) Symmetry breaking in the early mammalian embryo: the case for quantitative single-cell imaging analysis MHR-Molecular.

Human Reproduction 22:172-181.

Mohr, M.A., and Pantazis, P., (2016) Single neuron morphology in vivo with confined primed conversion.

Methods Cell Biology 133:125-38.

Figure selected as cover image for: Volume 1, Methods in Cell Biology, The Zebrafish: Cellular and Developmental Biology, Part A Cellular Biology 4th Edition.

Patents

Pantazis, P., Sonay, A.Y., Landfester, K., and Crespy, D., “Biodegradable second harmonic generating nanoprobes” E.P. patent pending (2016, EP 2016/191538). Among the top 20 innovations of ETH Zurich in 2016

Pantazis, P.,Pu, Y., Hong, J., Psaltis, D., and Fraser, S.E. “Second Harmonic Imaging Nanoprobes And Techniques For Use Thereof” U.S. patent granted (25 Oct 2016, US 9,476,830).

Pantazis, P., Culic-Viskota, J., Dempsey, W.P., and Fraser, S.E. “Functionalization of and use of functionalized second harmonic generating nanoprobes” U.S. patent granted (31 May 2016, US 9,352,055).

2015

Publications

Dempsey, W.P., Hodas, N.O., Ponti, A., and Pantazis, P., (2015) Determination of the source of SHG verniers in zebrafish skeletal muscle.

Scientific Reports 5:18119.

Dempsey, W.P., Georgieva, L., Helbling, P.M., Sonay, A.Y., Truong, T.V., Haffner, M., and Pantazis, P., (2015) In vivo single cell labeling by confined primed conversion.

Nature Methods 12:645–648.

Featured in 11 news outlets and scientific blogs among them: Bergman, F. (2015) Chameleon proteins make individual cells visible ETH Zurich News.

Invention W.O. patent pending (2014, WO 2014/147211)

E.P. patent pending (2014, EP 2014/055669) licensed to: DNAWrite, Cambridge/UK and Viventis Microscopy, Lausanne/CH.

Patents

Pantazis, P., Culic-Viskota, J., Dempsey, W.P., and Fraser, S.E. “Functionalization of and use of functionalized second harmonic generating nanoprobes” U.S. patent granted (29 Dec 2015, US 9,221,919).

Pantazis, P., Masmanidis, S., and Fraser, S.E. “Multipurpose Analysis Using Second Harmonic Generating Nanoprobes” U.S. patent granted (03 Feb 2015, US 8,945,471 B2).

2014

Publications

Pantazis, P., and Supatto W., (2014) Advances in whole-embryo imaging: a quantitative transition is underway.

Nature Reviews Molecular Cell Biology 15:327–39.

Dempsey, W.P., Qin, H., and Pantazis, P., (2014) In vivo cell tracking using PhOTO Zebrafish.

Methods Molecular Biology 1148:217-28.

Patents

Pantazis, P., Dempsey, W.P., Truong, T.V., Fraser, S.E., and Georgieva, L.“Method And Device To Achieve Spatially Confined Photointeraction At The Focal Volume Of A Microscope” W.O. patent pending (2014, WO 2014/147211).

Pantazis, P., Dempsey, W.P., Truong, T.V., Fraser, S.E., and Georgieva, L.“Method And Device To Achieve Spatially Confined Photointeraction At The Focal Volume Of A Microscope” E.P. patent pending (2014, EP 2014/055669).

2013

Publications

Mikut R., Dickmeis T., Driever W., Geurts P., Hamprecht F.A., Kausler B.X., Ledesma-Carbayo M.J., Marée R., Mikula K., Pantazis P., Ronneberger O., Santos A., Stotzka R., Strähle U., and Peyriéras N., (2013) Automated processing of zebrafish imaging data: A survey.

Zebrafish 10:401-21.

2012

Publications

Culic-Viskota, J., Dempsey, W.P., Fraser, S.E., and Pantazis, P., (2012) Surface functionalization of barium titanate SHG nanoprobes for in vivo imaging in zebrafish.

Nature Protocols 7:1618-33.

Dempsey, W.P., Fraser, S.E., and Pantazis, P.,(2012) PhOTO Zebrafish: A transgenic resource for in vivo lineage tracing during development and regeneration.

PLoS ONE 7:e32888.

Featured in: PhOTO zebrafish: a transgenic resource for in vivo lineage tracing during development and regeneration, Significance statement Global Medical Discovery August 25 2012.

Pantazis, P., and Bollenbach, T., (2012) Transcription factor kinetics and the emerging asymmetry in the early mammalian embryo.

Cell Cycle 11:2055-8.

Dempsey, W.P., Fraser, S.E., and Pantazis, P., (2012) SHG nanoprobes: Advancing harmonic imaging in biology.

Bioessays 34:351-60.

2011

Publications

Plachta, N., Bollenbach, T., Pease, S., Fraser, S.E., and Pantazis, P., (2011) Oct4 kinetics predict cell lineage patterning in the early mammalian embryo.

Nature Cell Biology 13:117-23.

Provided front cover of the journal and featured in: Zernicka-Goetz, M., (2011) Proclaiming fate in the early mouse embryo Nature Cell Biology 13:112-4.

Caneparo, L., Pantazis, P., Dempsey, W.P., and Fraser, S.E., (2011) Intercellular bridges in vertebrate gastrulation.

PLoS ONE 6:e20230.

2010

Publications

Pantazis, P., Maloney, J., Wu, D., and Fraser, S.E., (2010) Second Harmonic Generating (SHG) nanoprobes for in vivo imaging.

Proc Natl Acad Sci USA 107:14535-40.

Featured in: Cohen, B., (2010) Biological imaging: Beyond fluorescence Nature467:407-8; Evanko, D., (2010) Microscope harmonies Nature Methods7:779.

Tu, C., Ma, X., Pantazis, P., Kauzlarich, S., and Louie, A., (2010) Paramagnetic, silicon quantum dots for magnetic resonance and two photon imaging of macrophages.

J Am Chem Soc 132:2016-23.

Patents

Pantazis, P., Masmanidis, S., and Fraser, S.E. “Multipurpose Analysis Using Second Harmonic Generating Nanoprobes” W.O. patent pending (2010, WO 2010/090844).

2009

Publications

Pantazis, P., Pu, Y., Psaltis, D., and Fraser, S.E., (2009) Second Harmonic Generating (SHG) Nanoprobes: A new tool for biomedical imaging.

Proc. SPIE 7183:71831-5.

2008

Publications

Bollenbach, T., Pantazis, P., Kicheva, A., Boekel, C., Kruse, K., Gonzalez-Gaitan, M., and Juelicher, F., (2008) Precision of the Dpp gradient.

Development.135:1137-46.

Patents

Pantazis, P., Pu, Y., Hong, J., Psaltis, D., and Fraser, S.E. “Second Harmonic Imaging Nanoprobes And Techniques For Use Thereof” W.O. patent pending (2008, WO 2008/140584).

2007

Publications

Kicheva, A., Pantazis, P., Bollenbach, T., Kalaidzidis, Y., Bittig, T., Juelicher, F., and Gonzalez-Gaitan, M., (2007) Kinetics of morphogen gradient formation.

Science 315(5811):521-5.

Featured in: Kritikou, E., (2007) Reaching one’s range Nature Reviews Molecular Cell Biology8:181.

Pantazis, P., and González-Gaitán, M., (2007) Localized multiphoton photoactivation of paGFP in Drosophila wing imaginal discs.

J Biomed Opt 12:044004.

Bollenbach, T., Kruse, K., Pantazis, P., Gonzalez-Gaitan, M., Juelicher, F., (2007) Morphogen transport in epithelia.

Phys Rev E Stat Nonlin Soft Matter Phys. 75 (1 Pt 1) :011901.

2005

Publications

Bollenbach, T., Kruse, K., Pantazis, P., Gonzalez-Gaitan, M., and Juelicher, F., (2005) Robust formation of morphogen gradients.

Phys Rev Lett 94:018103(1-4).

Kruse, K., Pantazis, P., Bollenbach, T., Juelicher, F., and Gonzalez-Gaitan, M., (2004) Dpp gradient formation by Dynamin-dependent endocytosis: receptor trafficking and the diffusion model.

Development 131:4843-56.

2004

Publications

Dudu, V., Pantazis, P., and Gonzalez-Gaitan, M., (2004) Membrane traffic during embryonic development: epithelial formation, cell fate decisions and differentiation.

Curr Opin Cell Biol 16:407-414.

1999

Publications

Tsikas, D., Sandmann, J., Holzberg, D., Pantazis, P., Raida, M., and Frolich, J.C., (1999) Determination of S-nitrosoglutathione in human and rat plasma by high-performance liquid chromatography with fluorescence and ultraviolet absorbance detection after precolumn derivatization with o-phthalaldehyde.

Anal Biochem 273:32-40.

PANTAZIS LAB

Imperial College London

Publications and Patents

In Revision/Preparation

Yaganoglu, S., Helassa, N., Gaub, B.M., Welling, M.A., Shi, J., Müller, D.J., Török, K., and Pantazis, P., GenEPi: Piezo1 based

fluorescent reporter for visualizing mechanical stimuli with high spatiotemporal resolution.

Invention filed: E.P. patent pending (2016, EP 2016/191538).

​

​

​

Sonay, A.Y., Yaganoglu, S., Konantz, M., Teulon, C., Jiang, S., Behzadi, S., Crespy, D., Landfester, K., Roke, S., Lengerke, C., and Pantazis, P., Biodegradable harmonophores for targeted high-resolution in vivo tumor imaging.

Invention filed: E.P. patent pending (2016, EP 2016/191538). Nominated for the SPARK Award 2017.

​

​

2020

Publications

​

​

Welling, M., Kalyviotis, K., Pantazis, P., Primed Track: reliable volumetric single-cell tracking and lineage tracing of living specimen with dual-labeling approaches.

Bio-protocol. (Accepted)

​

Kalyviotis, K., Qin, H., Pantazis, P., (2020) Chapter 19 - PhOTO zebrafish and primed conversion: advancing the mechanistic view of development and disease. In: Gerlai RT, editor. Behavioral and Neural Genetics of Zebrafish.

Academic Press. p. 309-22. doi:10.1016/B978-0-12-817528-6.00019-X

​

2019

Publications

​

Welling, M.A., Mohr, M.A., Ponti, A., Sabater, L.R., Boni, A., Kitazawa, Y., Liberali, P., Peters, A., Pelczar, P., and Pantazis, P. (2019)

Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins.

eLife 8, doi:10.7554/eLife.44491

​

2018

Publications

​

Slenders, E., Bové, H., Urbain, M., Mugnier, Y., Sonay. A.Y., Pantazis, P., Bonacina, L., Vanden Berghe, P., vandeVen, M., and Ameloot, M., (2018) Image Correlation Spectroscopy with Second Harmonic Generating Nanoparticles in Suspension and in Cells.

J Phys Chem Lett. 20:6112-6118.

Sugiyama, N., Sonay, A.Y., Tussiwand, R., Cohen, B., and Pantazis, P., (2018) Effective Labeling of Primary Somatic Stem Cells with

BaTiO3 Nanocrystals for Second Harmonic Generation Imaging.

Small 8:1703386.

Mohr, M.A., and Pantazis, P., (2018) Primed conversion: the new kid on the block for photoconversion.

Chemistry 33:8268-8274.

Nienhaus, K., Mohr, M.A., Kobitski, A,Y., Sabater, L.R., Obara, C.J., Lippincott Schwartz, J., Nienhaus, G.U., and Pantazis, P. (2018)

Primed Green to Red Photoconversion of Fluorescent Proteins Occurs via a Triplet State.

Biophysical Journal 114:533a.

Patents

Pantazis, P., Dempsey, W.P., Truong, T.V., Fraser, S.E., and Georgieva, L. “Method And Device To Achieve Spatially Confined Photointeraction At The Focal Volume Of A Microscope” U.P. patent granted (15 May 2018, US 90,971,136).

Pantazis, P., Pu, Y., Hong, J., Psaltis, D., and Fraser, S.E. “Second Harmonic Imaging Nanoprobes And Techniques For Use Thereof” U.S. patent granted (20 Feb 2018, US 9,971,136).

2017

Publications

Nugraha, B., Mohr, M.A., Ponti, A., Emmert, M.Y., Weibel, F., Hoerstrup, S.P., Moll, S., Certa, U., Prunotto, M., and Pantazis, P., (2017)

Monitoring and manipulating cellular crosstalk during kidney fibrosis inside a 3D in vitro co-culture.

Scientific Reports 7:14490.

​

Mohr, M.A., Kobitski, A.Yu., Sabater, L.R. Nienhaus, K., Obara, C.J, Lippincott Schwartz, J., Nienhaus, G.U., and Pantazis, P., (2017)

Rational Engineering of Photoconvertible Fluorescent Proteins for Dual Color Fluorescence NanoscopyEnabled by a Triplet State Mechanism of Primed Conversion.

Angew. Chem. Int. Ed. 56:11628 11633.

Featured in: Bergman, F. (2017) Red fluorescence in two steps ETH Zurich News.

Invention filed: U.S. provisional patent submitted (2017, Application Serial No. 62/446,023).

Zhang,W., Lohman, A.W., Zhuravlova, Y., Lu, X., Wiens, M.D., Hoi, H., Yaganoglu, S.,Mohr, M.A., Kitova, E.N., Klassen, J.S., Pantazis, P., Thompson, J.R., and Campbell, R.E., (2017) Optogenetic control with a photocleavable protein.

Nature Methods 14:391–394.

Sonay, A.Y., and Pantazis, P., (2017) Bioinspired Second Harmonic Generation.

Proc. SPIE 10411, Clinical and Preclinical Optical Diagnostics,104110D.

Patents

Pantazis, P.and Yaganoglu, S., “Piezo1-based fluorescent reporter” E.P. patent pending (2017, EP 2017/17210479.6).

Mohr, M.A.,and Pantazis, P., “Engineered photoconvertible fluorescent proteins (pcFPs) for primed conversion” U.S. patent pending (2017, US 62/446,023).

​

2016

Publications

​

Mohr, M.A., Argast, P., and Pantazis, P., (2016) Labeling cellular structures in vivo using confined primed conversion of photoconvertible fluorescent proteins.

Nature Protocols 11:2419-2431.

Invention W.O. patent pending (2014, WO 2014/147211)

E.P. patent pending (2014, EP 2014/055669) licensed to: AHF Analysentechnik, Tübingen/Germany.

Welling, M., Ponti, A., and Pantazis, P., (2016) Symmetry breaking in the early mammalian embryo: the case for quantitative single-cell imaging analysis MHR-Molecular.

Human Reproduction 22:172-181.

Mohr, M.A., and Pantazis, P., (2016) Single neuron morphology in vivo with confined primed conversion.

Methods Cell Biology 133:125-38.

Figure selected as cover image for: Volume 1, Methods in Cell Biology, The Zebrafish: Cellular and Developmental Biology, Part A Cellular Biology 4th Edition.

​

Patents