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Publications and Patents
2023
Publications
Yaganoglu, S., Kalyviotis, K., Vagena-Pantoula, C., Jülich, D., Gaub, B.M., Welling, M., Lopes, T., Lachowski, D., Tang, S.S., Del Rio Hernandez, A., Salem, V., Müller, D.J., Holley, S.A., Vermot, J., Shi, J., Helassa, N., Török, K. and Pantazis, P., (2023). Highly specific and non-invasive imaging of Piezo1-dependent activity across scales using GenEPi.
Nat Commun. 2023 Jul 19;14(1):4352. doi: 10.1038/s41467-023-40134-y.
2024
Publications
Kalyviotis, K., Pantazis, P., (2024). Primed conversion: The emerging player of precise and nontoxic photoconversion.J Microsc. 2024 Nov;296(2):154-161. doi: 10.1111/jmi.13244.
Guo, K., Kalyviotis, K., Pantazis, P., Rowlands, C.J., (2024). Hyperspectral oblique plane microscopy enables spontaneous, label-free imaging of biological dynamic processes in live animals.
Proc Natl Acad Sci USA 2024 Oct 22;121(43):e2404232121. doi: 10.1073/pnas.2404232121.
Ulucay, E., Bollier, P., Spilioti, M., Sonay, A.Y., Kalyviotis, K. and Pantazis, P., (2024). Synthesis and Functionalization of Biodegradable Second Harmonic Generation Nanoprobes for Cell Targeting.
Curr Protoc. 2024 Jul;4(7):e1089. doi: 10.1002/cpz1.1089.
Suba, K., Patel,Y., Alonso, A.M., Hansen, B., Xu, X., Roberts, A., Norton, M., Chung, K., Shrewsbury, J.V., Kwok, R., Kalogianni, V., Chen, S., Liu, X., Kalyviotis, K., Rutter, G.A., Jones, B.J., Miniion, J., Owen, B.M., Pantazis, P., Distaso, W., Drucker, D.J., Tan, T.M., Bloom, S.R., Murphy, K.G., Salem, V., (2024). Intra-islet glucagon signalling regulates beta-cell connectivity, first-phase insulin secretion and glucose homoeostasis.
Mol Metab. 2024 Jul:85:101947. doi: 10.1016/j.molmet.2024.101947.
2021
Publications
Sonay, A. Y., Kalyviotis, K., Yaganoglu, S., Unsal, A., Konantz, M., Teulon, C., Lieberwirth, I., Sieber, S., Jiang, S., Behzadi, S., Crespy, D., Landfester, K., Roke, S., Lengerke, C. and Pantazis, P., (2021). Biodegradable Harmonophores for Targeted High-Resolution In Vivo Tumor Imaging.
ACS Nano 15(3): 4144-4154. doi: 10.1021/acsnano.0c10634
Fang, C., Drobizhev, M., Ng, H.L. and Pantazis, P., (2021). Mechanisms of Fluorescent Proteins.
Frontiers in Molecular Biosciences 8, 701523. doi.org/10.3389/fmolb.2021.701523
Patents
Pantazis, P., Sonay, A.Y., Landfester, K., and Crespy, D., “Biodegradable second harmonic generating nanoprobes” E.P. patent granted (12 November 2021, EP 3518986).
2020
Publications
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Welling, M., Kalyviotis, K., Pantazis, P., (2020). Primed Track: reliable volumetric single-cell tracking and lineage tracing of living specimen with dual-labeling approaches.
Bio-protocol. doi: 10.21769/BioProtoc.3645.​
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Kalyviotis, K., Qin, H., Pantazis, P., (2020) Chapter 19 - PhOTO zebrafish and primed conversion: advancing the mechanistic view of development and disease. In: Gerlai RT, editor. Behavioral and Neural Genetics of Zebrafish.
Academic Press. p. 309-22. doi:10.1016/B978-0-12-817528-6.00019-X
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Malkinson, G., Mahou, P., Chaudan, É., Gacoin, T., Sonay, A. Y., Pantazis, P., Beaurepaire, E., Supatto, W., (2020) Fast In Vivo Imaging of SHG Nanoprobes with Multiphoton Light-Sheet Microscopy.
ACS Photonics 2020, 7 (4), 1036-1049.
2019
Publications
Welling, M.A., Mohr, M.A., Ponti, A., Sabater, L.R., Boni, A., Kitazawa, Y., Liberali, P., Peters, A., Pelczar, P., and Pantazis, P. (2019)
Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins.
eLife 8, doi:10.7554/eLife.44491
2018
Publications
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Slenders, E., Bové, H., Urbain, M., Mugnier, Y., Sonay. A.Y., Pantazis, P., Bonacina, L., Vanden Berghe, P., vandeVen, M., and Ameloot, M., (2018) Image Correlation Spectroscopy with Second Harmonic Generating Nanoparticles in Suspension and in Cells.
J Phys Chem Lett. 20:6112-6118.
Sugiyama, N., Sonay, A.Y., Tussiwand, R., Cohen, B., and Pantazis, P., (2018) Effective Labeling of Primary Somatic Stem Cells with
BaTiO3 Nanocrystals for Second Harmonic Generation Imaging.
Small 8:1703386.
Mohr, M.A., and Pantazis, P., (2018) Primed conversion: the new kid on the block for photoconversion.
Chemistry 33:8268-8274.
Nienhaus, K., Mohr, M.A., Kobitski, A,Y., Sabater, L.R., Obara, C.J., Lippincott Schwartz, J., Nienhaus, G.U., and Pantazis, P. (2018)
Primed Green to Red Photoconversion of Fluorescent Proteins Occurs via a Triplet State.
Biophysical Journal 114:533a.
Patents
Pantazis, P., Dempsey, W.P., Truong, T.V., Fraser, S.E., and Georgieva, L. “Method And Device To Achieve Spatially Confined Photointeraction At The Focal Volume Of A Microscope” U.P. patent granted (15 May 2018, US 90,971,136).
Pantazis, P., Pu, Y., Hong, J., Psaltis, D., and Fraser, S.E. “Second Harmonic Imaging Nanoprobes And Techniques For Use Thereof” U.S. patent granted (20 Feb 2018, US 9,971,136).
2017
Publications
Nugraha, B., Mohr, M.A., Ponti, A., Emmert, M.Y., Weibel, F., Hoerstrup, S.P., Moll, S., Certa, U., Prunotto, M., and Pantazis, P., (2017)
Monitoring and manipulating cellular crosstalk during kidney fibrosis inside a 3D in vitro co-culture.
Scientific Reports 7:14490.
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Mohr, M.A., Kobitski, A.Yu., Sabater, L.R. Nienhaus, K., Obara, C.J, Lippincott Schwartz, J., Nienhaus, G.U., and Pantazis, P., (2017)
Rational Engineering of Photoconvertible Fluorescent Proteins for Dual Color Fluorescence NanoscopyEnabled by a Triplet State Mechanism of Primed Conversion.
Angew. Chem. Int. Ed. 56:11628 11633.
Featured in: Bergman, F. (2017) Red fluorescence in two steps ETH Zurich News.
Invention filed: U.S. provisional patent submitted (2017, Application Serial No. 62/446,023).
Zhang,W., Lohman, A.W., Zhuravlova, Y., Lu, X., Wiens, M.D., Hoi, H., Yaganoglu, S.,Mohr, M.A., Kitova, E.N., Klassen, J.S., Pantazis, P., Thompson, J.R., and Campbell, R.E., (2017) Optogenetic control with a photocleavable protein.
Nature Methods 14:391–394.
Sonay, A.Y., and Pantazis, P., (2017) Bioinspired Second Harmonic Generation.
Proc. SPIE 10411, Clinical and Preclinical Optical Diagnostics,104110D.
Patents
Pantazis, P.and Yaganoglu, S., “Piezo1-based fluorescent reporter” E.P. patent pending (2017, EP 2017/17210479.6).
Mohr, M.A.,and Pantazis, P., “Engineered photoconvertible fluorescent proteins (pcFPs) for primed conversion” U.S. patent pending (2017, US 62/446,023).
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2016
Publications
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Mohr, M.A., Argast, P., and Pantazis, P., (2016) Labeling cellular structures in vivo using confined primed conversion of photoconvertible fluorescent proteins.
Nature Protocols 11:2419-2431.
Invention W.O. patent pending (2014, WO 2014/147211)
E.P. patent pending (2014, EP 2014/055669) licensed to: AHF Analysentechnik, Tübingen/Germany.
Welling, M., Ponti, A., and Pantazis, P., (2016) Symmetry breaking in the early mammalian embryo: the case for quantitative single-cell imaging analysis MHR-Molecular.
Human Reproduction 22:172-181.
Mohr, M.A., and Pantazis, P., (2016) Single neuron morphology in vivo with confined primed conversion.
Methods Cell Biology 133:125-38.
Figure selected as cover image for: Volume 1, Methods in Cell Biology, The Zebrafish: Cellular and Developmental Biology, Part A Cellular Biology 4th Edition.
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Patents
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Pantazis, P., Sonay, A.Y., Landfester, K., and Crespy, D., “Biodegradable second harmonic generating nanoprobes” E.P. patent pending (2016, EP 2016/191538). Among the top 20 innovations of ETH Zurich in 2016
Pantazis, P.,Pu, Y., Hong, J., Psaltis, D., and Fraser, S.E. “Second Harmonic Imaging Nanoprobes And Techniques For Use Thereof” U.S. patent granted (25 Oct 2016, US 9,476,830).
Pantazis, P., Culic-Viskota, J., Dempsey, W.P., and Fraser, S.E. “Functionalization of and use of functionalized second harmonic generating nanoprobes” U.S. patent granted (31 May 2016, US 9,352,055).
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2015
Publications
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Dempsey, W.P., Hodas, N.O., Ponti, A., and Pantazis, P., (2015) Determination of the source of SHG verniers in zebrafish skeletal muscle.
Scientific Reports 5:18119.
Dempsey, W.P., Georgieva, L., Helbling, P.M., Sonay, A.Y., Truong, T.V., Haffner, M., and Pantazis, P., (2015) In vivo single cell labeling by confined primed conversion.
Nature Methods 12:645–648.
Featured in 11 news outlets and scientific blogs among them: Bergman, F. (2015) Chameleon proteins make individual cells visible ETH Zurich News.
Invention W.O. patent pending (2014, WO 2014/147211)
E.P. patent pending (2014, EP 2014/055669) licensed to: DNAWrite, Cambridge/UK and Viventis Microscopy, Lausanne/CH.
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Patents
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Pantazis, P., Culic-Viskota, J., Dempsey, W.P., and Fraser, S.E. “Functionalization of and use of functionalized second harmonic generating nanoprobes” U.S. patent granted (29 Dec 2015, US 9,221,919).
Pantazis, P., Masmanidis, S., and Fraser, S.E. “Multipurpose Analysis Using Second Harmonic Generating Nanoprobes” U.S. patent granted (03 Feb 2015, US 8,945,471 B2).
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2014
Publications
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Pantazis, P., and Supatto W., (2014) Advances in whole-embryo imaging: a quantitative transition is underway.
Nature Reviews Molecular Cell Biology 15:327–39.
Dempsey, W.P., Qin, H., and Pantazis, P., (2014) In vivo cell tracking using PhOTO Zebrafish.
Methods Molecular Biology 1148:217-28.
Patents
Pantazis, P., Dempsey, W.P., Truong, T.V., Fraser, S.E., and Georgieva, L.“Method And Device To Achieve Spatially Confined Photointeraction At The Focal Volume Of A Microscope” W.O. patent pending (2014, WO 2014/147211).
Pantazis, P., Dempsey, W.P., Truong, T.V., Fraser, S.E., and Georgieva, L.“Method And Device To Achieve Spatially Confined Photointeraction At The Focal Volume Of A Microscope” E.P. patent pending (2014, EP 2014/055669).
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2013
Publications
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Mikut R., Dickmeis T., Driever W., Geurts P., Hamprecht F.A., Kausler B.X., Ledesma-Carbayo M.J., Marée R., Mikula K., Pantazis P., Ronneberger O., Santos A., Stotzka R., Strähle U., and Peyriéras N., (2013) Automated processing of zebrafish imaging data: A survey.
Zebrafish 10:401-21.
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2012
Publications
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Culic-Viskota, J., Dempsey, W.P., Fraser, S.E., and Pantazis, P., (2012) Surface functionalization of barium titanate SHG nanoprobes for in vivo imaging in zebrafish.
Nature Protocols 7:1618-33.
Dempsey, W.P., Fraser, S.E., and Pantazis, P.,(2012) PhOTO Zebrafish: A transgenic resource for in vivo lineage tracing during development and regeneration.
PLoS ONE 7:e32888.
Featured in: PhOTO zebrafish: a transgenic resource for in vivo lineage tracing during development and regeneration, Significance statement Global Medical Discovery August 25 2012.
Pantazis, P., and Bollenbach, T., (2012) Transcription factor kinetics and the emerging asymmetry in the early mammalian embryo.
Cell Cycle 11:2055-8.
Dempsey, W.P., Fraser, S.E., and Pantazis, P., (2012) SHG nanoprobes: Advancing harmonic imaging in biology.
Bioessays 34:351-60.
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2011
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Publications
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Plachta, N., Bollenbach, T., Pease, S., Fraser, S.E., and Pantazis, P., (2011) Oct4 kinetics predict cell lineage patterning in the early mammalian embryo.
Nature Cell Biology 13:117-23.
Provided front cover of the journal and featured in: Zernicka-Goetz, M., (2011) Proclaiming fate in the early mouse embryo Nature Cell Biology 13:112-4.
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Caneparo, L., Pantazis, P., Dempsey, W.P., and Fraser, S.E., (2011) Intercellular bridges in vertebrate gastrulation.
PLoS ONE 6:e20230.
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2010
Publications
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Pantazis, P., Maloney, J., Wu, D., and Fraser, S.E., (2010) Second Harmonic Generating (SHG) nanoprobes for in vivo imaging.
Proc Natl Acad Sci USA 107:14535-40.
Featured in: Cohen, B., (2010) Biological imaging: Beyond fluorescence Nature467:407-8; Evanko, D., (2010) Microscope harmonies Nature Methods7:779.
Tu, C., Ma, X., Pantazis, P., Kauzlarich, S., and Louie, A., (2010) Paramagnetic, silicon quantum dots for magnetic resonance and two photon imaging of macrophages.
J Am Chem Soc 132:2016-23.
Patents
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Pantazis, P., Masmanidis, S., and Fraser, S.E. “Multipurpose Analysis Using Second Harmonic Generating Nanoprobes” W.O. patent pending (2010, WO 2010/090844).
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2009
Publications
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Pantazis, P., Pu, Y., Psaltis, D., and Fraser, S.E., (2009) Second Harmonic Generating (SHG) Nanoprobes: A new tool for biomedical imaging.
Proc. SPIE 7183:71831-5.
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2008
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Publications
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Bollenbach, T., Pantazis, P., Kicheva, A., Boekel, C., Kruse, K., Gonzalez-Gaitan, M., and Juelicher, F., (2008) Precision of the Dpp gradient.
Development.135:1137-46.
Patents
Pantazis, P., Pu, Y., Hong, J., Psaltis, D., and Fraser, S.E. “Second Harmonic Imaging Nanoprobes And Techniques For Use Thereof” W.O. patent pending (2008, WO 2008/140584).
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2007
Publications
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Kicheva, A., Pantazis, P., Bollenbach, T., Kalaidzidis, Y., Bittig, T., Juelicher, F., and Gonzalez-Gaitan, M., (2007) Kinetics of morphogen gradient formation.
Science 315(5811):521-5.
Featured in: Kritikou, E., (2007) Reaching one’s range Nature Reviews Molecular Cell Biology8:181.
Pantazis, P., and González-Gaitán, M., (2007) Localized multiphoton photoactivation of paGFP in Drosophila wing imaginal discs.
J Biomed Opt 12:044004.
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Bollenbach, T., Kruse, K., Pantazis, P., Gonzalez-Gaitan, M., Juelicher, F., (2007) Morphogen transport in epithelia.
Phys Rev E Stat Nonlin Soft Matter Phys. 75 (1 Pt 1) :011901.​
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2005
Publications
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Bollenbach, T., Kruse, K., Pantazis, P., Gonzalez-Gaitan, M., and Juelicher, F., (2005) Robust formation of morphogen gradients.
Phys Rev Lett 94:018103(1-4).
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Kruse, K., Pantazis, P., Bollenbach, T., Juelicher, F., and Gonzalez-Gaitan, M., (2004) Dpp gradient formation by Dynamin-dependent endocytosis: receptor trafficking and the diffusion model.
Development 131:4843-56.
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2004
Publications
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Dudu, V., Pantazis, P., and Gonzalez-Gaitan, M., (2004) Membrane traffic during embryonic development: epithelial formation, cell fate decisions and differentiation.
Curr Opin Cell Biol 16:407-414.
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1999
Publications
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Tsikas, D., Sandmann, J., Holzberg, D., Pantazis, P., Raida, M., and Frolich, J.C., (1999) Determination of S-nitrosoglutathione in human and rat plasma by high-performance liquid chromatography with fluorescence and ultraviolet absorbance detection after precolumn derivatization with o-phthalaldehyde.
Anal Biochem 273:32-40.
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