©2019

P A N T A Z I S  L A B.

PRIMED
CONVERSION
GenEPi
BIOHARMONOPHORES

Primed Conversion

The PANTAZIS LAB captures the chemical and mechanical dynamics 

of development and disease by using cutting-edge approaches to live imaging.

 

 

Mechanosensing is a ubiquitous process to translate external mechanical stimuli into biological responses during development, homeostasis, and disease. However, non-invasive investigation of cellular mechanosensing in complex and intact live tissue remains challenging. We developed GenEPi, a genetically-encoded fluorescent intensiometric reporter for mechanical stimuli based on Piezo1, an essential mechanosensitive ion channel found in vertebrates. GenEPi has high specificity and spatiotemporal resolution, enabling the precise understanding how physical forces drive biological function during, for instance, tissue growth, wound healing and cancer cell invasion.

 

Optical imaging probes have played a major role in detecting and monitoring of a variety of diseases. Ideally, contrast agents for biomedical applications should degrade without any long-term toxicological consequences to the patient. We developed biodegradable imaging probes, termed bioHarmonophores, that generate a strong nonlinear signal. These reporters can target single cancer cells with high detection sensitivity in vivo, enabling an innovative precision imaging approach to cancer diagnostics and therapy.

 

Accurate reconstruction of the dynamics of individual molecules, intracellular organelles, entire cells and multicellular structures is essential for inferring development and disease progression. Green-to-red photoconvertible fluorescent proteins (pcFPs) have been extensively used as molecular highlighters, yet precise photoconversion was limited to thin samples due to the axially unconfined nature of the violet conversion light. Recently, we reported a novel way to photoconvert pcFPs, termed primed conversion, which allows precise labelling in 3D environments and even subcellular compartments. Primed conversion has also been successfully extended to super-resolution imaging with low phototoxicty and optogenetics, opening the path to functional manipulation of cells at single cell resolution.

GenEPi

Forse Sensor

Bioharmonophores